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In some situations when the maturity and integrity of the spermatozoa requires confirmation, the laboratory performs the PICSI procedure. In this video it is shown how spermatozoa included in the sperm preparation are exposed to a plate containing hyaluron. Mature sperm are known to adhere to hyaluron while immature ones swim through without any adherence to the hyaluron spot. Before performing ICSI spermatozoa are loaded from the PICSI dish and placed into PVP before injection inside the oocyte.