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January 05, 2015
Category : Laboratory Videos

Sperm on PVP

When ICSI is required, the motile spermatozoa have to be placed in a culture media containing a viscous substance called PVP. This media reduces the speed of the sperm so it can be immobilized and loaded into the injection micropipette. The immobilization process also activates the spermatozoa so it can perform a normal fertilization of the oocyte once it is placed in the oocyte cytoplasm.