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September 04, 2014
Category : Laboratory Videos

ICSI Procedure

After oocytes are retrieved and cultured for at least four hours they are denuded from the surrounding cells and injected with one spermatozoon for fertilization.
This process is performed in a micromanipulator using precision tools (micropipetes). The video shows one holding and one injection pipette at a 200X magnification. The oocyte is hold into position with negative pressure and the sperm, previously loaded in the injection micropipette, is placed inside the oocyte after the zona pellucida and the cytoplasmic membrane are penetrated.